The ratio between control lysate signal / non-specific signal should be greater than 2. no. Push the screw down and turn it clockwise to lock the plate. HTRF Signal + 16 µL Standard or Sample 4 µL of pre-mixed TNFα Antibodies ADD READ ANALYSE Incubate 2 hours* at room temperature *Following incubation, the signal remains stable over a period of 24 hours. Make sure to use the set-up for Eu Cryptate. Better sensitivity provides better statistic, less variability among replicates, and a larger assay window. 14 seconds for a 384-well plate. NON-SPECIFIC BINDING TOTAL BINDINGCover the plate with a plate sealer. High-end TR-FRET/HTRF® performance: certified ®to measure HTRF assays in both black or white plates, the reader guarantees no compromises in any HTRF assay. Lysates are then transferred to the assay plate for the detection of Phospho-AKT-3 (Ser473) by HTRF. 3. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. FOR HTRF CERTIFIED. Setu CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified. It also integrates with the powerful SoftMax Pro Software for. 96-well culture plate+ 4 µL HTRF pre-mixed antibodies. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. HTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. Some vendors hold the entire market share for not only a. Compared to ELISA technology, MSD assays: Require far less sample. SV detection plate. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. It can also be further streamlinedFOR HTRF CERTIFIED READER. These are the different kinetic modes on BMG LABTECH microplate readers. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. The ratio of the two fluorescence TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: + 10 µL lysis buffer ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. Read & analyse. 1. Read & analyse. These plates can be read on all HTRF certified readers, thus making their implementation in. Lyse cells Read on an HTRF compatible reader . Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Figure 3. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. FOR HTRF CERTIFIED READER . For more information about set-up and compatible HTRF® readers,The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Lysates are then transferred to the assay plate for the detection of Phospho-VASP (Ser239) by HTRF. Remove the plate seal and read on an HTRF compatible reader. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. + 4 µL HTRF ® pre-mixed antibo dies 2. It features a modular design that allows users to add or change detection cartridges according to their needs. You can count on our plate readers to bring you the performance, reliability, consistent results, and the best user experience available—across a multitude of applications and a range of detection options. SERIAL DILUTIONS . Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. White 384-well low volume plate Make sure to use the set-up for Eu3+ Cryptate. 96-well culture plateFOR HTRF CERTIFIED READER For more information about HTRF. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. It features a modular design that allows users to add or change detection cartridges according to their needs. Its. FOR HTRF CERTIFIED READER For more. Results for HTRF Reader Control Kit with black and white microplates. For more ® information about HTRF compatible readers and for set-up recommendations, please. According to the manufacturers protocol, this protein assay is linear in the range of 0. • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readersHTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. The SpectraMax i3x and iD5 readers can be equipped with an HTRF-certified detection cartridge or an HTRF-certified detection system, respectively. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Thanks to HTRF signal stability, the assay plate may be used to check several instruments, or. The samples were each placed in a well of a 24-well plate, covered with 1 ml of culture medium, and incubated for a total of 17 days. • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readersONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Step 1 Step 2 Step 3 Step 4 Step 5. Plate 25 μL of cells in. Transfer 5 µL of cell supernatant from the 96-well plate to a 384-small volume (SV) white detection plate . FOR HTRF CERTIFIED. Plates can be loaded manually or plates can be loaded using 20 or 50-plate free access magazines which fit in a stacker. FOR HTRF CERTIFIED READER. Taking these considerations into account, Cisbio’s 96w low volume and PKI’s 96w & 384w microplates have been carefully designed to provide the best signal detection environment possible to all HTRF assays. FOR HTRF CERTIFIED READERFOR HTRF CERTIFIED READER. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Microplate Readers and Reader Accessories. Page 30 4. PerkinElmer. In this case the Z´(pronounced Z prime) value comes to mind. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. Cells in each image are identified by SoftMax Pro Software and cell-by-cellHTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. Experimental details• HTRF®-Certified Reader **. VICTOR Nivo Multimode Plate Reader • Compact and lightweight • High performance for routine applications, including biochemical and cell-based assays • All major detection. Signals are detected at two different wavelengths (typically 620 nm and 665 nm), which are used to. Step 1 Step 2 Step 3 Step 4 Step 5 Step 6. It can also be further streamlined toLysates are then transferred ®to the assay plate for the detection of Total TYK2 by HTRF reagents. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:The PHERAstar FS is an advanced HTS plate reader designed for all HTRF assays and features the following: All microplate formats up to 3456-well. Read & analyse. Certified for use with Alpha, LANCE ® or DELFIA ®. HTRF certified . They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. For more information about HTRF. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Incubate overnight at RT. The PHERAstar ® FSX is our most sensitive multi-mode reader for high-throughput screening, combining this sensitivity with fast read times. 96-well culture plateHowever, time-resolved fluorescence (TRF & TR-FRET) assays are used in conjunction with fluorophores that have a longer half-life. Remove the plate sealer and read on an HTRF® compatible reader . Plus . * For HTRF microplate recommendations, please visit . Plate 25 μL of cells in. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. PROTOCOL AT A GLANCE Do not pre-mix the d2 and Cryptate solutions prior to dispensing. The measurement of HTRF assays is predominantly performed on microplate readers. The optical systems use two scanning All the benefits of HTRF with ELISA plate ease of use. Bench top solutions for cell based assays. SpectraMax® M4 Multi-Mode Microplate Reader MLDVM4 SpectraMax® M5 Multi-Mode Microplate Reader MLDVM5 SpectraMax® M5e Multi-Mode Microplate Reader (HTRF certified) MLDVM5E To prevent evaporation during assay incubation steps, we recommend the use of TopSeal-A PLUS, a clear adhesive seal for microplate. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™- certified. The assay was prepared in both white and black plates and incubated >18 hours. Incubate overnight at RT. Its unique optical design and instant polarizer switching provide the smallest mP standard deviation in any assay. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. VICTOR® Nivo™ multimode plate reader is the smallest plate reader on the. • HTRF®-Certified Reader **. Lysates are then transferred to the assay plate for the detection of total-RET by HTRF. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. a one- plate assay protocol. Dedicated UV-laser excitation. The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The ratio of the two fluorescence Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. Discover our high performance reader. spectramax paradigm system, htrf reagents, spectramax i3 and paradigm readers, HTRF assay, htrf certifiedLysates are then transferred ®to the assay plate for the detection of Total CHK2 by HTRF reagents. protocol with suspension cells or with a. CLARIOstar is equipped with a specific optical device which enables the measurement of. The VICTOR Nivo is a HTRF certified microplate reader. Both readers demonstrated their ability to detect IP1 concentrations according to Cisbio’s acceptance criteria. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. An HTRF assay measures the fluorescence energy transfer between molecules in close proximity. For more ® information about HTRF compatible readers and for set-up recommendations, please. HTRF® 96-well low volume plate Ref# 66PL96001 * HTRF® 384-well low volume plate Ref 66PL384025 * Non-binding 96-well black plate HTRF®-Certified Reader **. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. 96-well culture plateFor each fluorescent ligand concentration, the nonspecific binding was determined by adding a large excess of unlabeled spiperone. It is used as a positive control for Gs applications ( biological models validation,Detection of Total CDK12 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. * For HTRF microplate recommendations, please visit . Simply place the module into one of the five module bays and read the assay. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: 25 µL cells ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. After an overnight incubation, the plate is washed 3 times. EGFR protein (PV3872, 0. * For HTRF microplate recommendations, please visit Table 1 Instrument settings used for HTRF with terbium donor and red acceptor. Reader. ** The Detection Buffer is used to prepare working solutions of acceptor and donor reagents. The assay is then ready to be read on an HTRF compatible or certified microplate reader. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. FOR HTRF CERTIFIED READER. For more information about HTRF ®Seal the plate and incubate for Overnight. FOR HTRF CERTIFIED READER For. Plate 25 μL of cells in. Time-resolved fluorescence was measured on the SpectraMax Multi-Mode Microplate Readers (see Table 2 for instrument settings). Dual-Luciferase® (DLR™) gene assays. Seal the plate and incubate 3 hours at RT . Make sure the setup for Eu3+ Cryptate is used. Flexibility combined with the best sensitivity of its class, and our newly developed Enhanced Dynamic Range technology, make it the ideal reader for assay development. The HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. But sometimes these parameters are not sufficient enough. NanoQuant Plate™ allows rapid, straightforward measurement of 16 x 2 µl samples simultaneously without calibration. For more information about HTRF ®Detection of Total BRD4 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. 5% and an increased Z’ value of 0. Make sure the setup for Tb Cryptate is used. For fluorescent assays, black plates absorb light and reduce background and crosstalk. They can be used in a no-transfer. For more information about HTRF ®Highly compatible plates for HTRF. The new ligands are divided into four groups according to their chemical structures and/or interactions with YTHDC1 (Table 1 and Fig. For more information about microplate recommendations, please visit our website STORAGE AND STABILITYDetection. Utilizing HTRF (Homogeneous Time-resolved Fluorescence) technology, the assay enables simple and rapid characterization of compound and antibody blockers in a high throughput format. The PHERAstar FS is an advanced HTS plate reader designed for all HTRF assays and features the following: All microplate formats up to 3456-well. The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. For more information about HTRF. The optional HTRF-certified filter-based module may be. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. The ratio for each well must be calculated individually. Reagents were dispensed following Cisbio’s ligand binding protocol in 384-well format (white plates). Sensitive, accurate and reliable detection is critical across cell, protein and genomic analysis applications in drug discovery and development. Here’s how it works: we come to you with over 30 years of plate reader instrument development experience. • HTRF cAMP HiRange Assay Kit (Cisbio Bioassays, France) • 96-well half area plates (white) (Greiner Bio-One, Germany) • 384-well small volume plates (white and black) (Greiner Bio-One, Germany) HTRF RCK The HTRF RCK is used to confirm the general suitability of a reader for HTRF assays, as well as set up and performance validation. Example assays are ELISAs, DNA and protein concentration determination, steady-state dose response curves, ADP-Glo™ (Promega), Transcreener™ GDP fluorescent polarization. Its. The SpectraMax i3x and iD5 readers can be equipped with an HTRF-certified detection cartridge or an HTRF-certified detection system, respectively. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. The Dual-Luciferase ® Reporter Assay or DLR is widely used to study gene transcription and regulation. In addition, stacker. Incubate overnight at RT. For more information about HTRF. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. Lysates are then transferred to the assay plate for the detection of total-BTK by HTRF. FOR HTRF CERTIFIED READER. Lysates are then transferred to the assay plate for the detection of Phospho-AKT-2 (Ser473) by HTRF. FOR HTRF CERTIFIED. The EnVision XCite Plate Reader provides exceptional sensitivity and speed with minimal downtime - backed by our global network of application scientists and engineers. 96-well or 384-well small volume (SV) detection microplates - For more information about microplate recommendations, please. Plate 25 μL of cells in. It can also be further streamlined to a one-. It combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background. Designed to the highest standards to support your drug discovery research, Corning ® and Falcon ® microplates are ideal for drug discovery applications, including assay prep, assay development, and HTS and automation. • HTRF®-Certified Reader. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. See the Cytation 5 Operator’s Manual for more information and instructions. microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. Lysates are then transferred to the assay plate for the detection of total-STING by HTRF. This protocol gives the cells viability and confluence to be monitored. 4. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. HTRF plate reader. No washing steps are required. • HTRF®-Certified Reader **. Read & analyse. FOR HTRF CERTIFIED READER. For more ® information about HTRF compatible readers and for set-up recommendations. FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations, please visit our. and Canaa -8-963-4567. If not, our scientific team will help you choose the instrument that best suits your needs and budget. Lysates are then transferred to the assay plate for the detection of total-EGFR by HTRF. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. FOR HTRF CERTIFIED READER. The VICTOR Nivo. This protocol, HTS designed, allows miniaturization while. Incubate overnight at RT. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. HTRF is a versatile TR-FRET technology developed by Cisbio for detecting biomolecular interactions. FlexStation® 3 Multi-Mode Microplate Reader. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. • User-upgradable microplate reader in under 2 minutes • Array of detection cartridge applications at your fingertips. FOR HTRF CERTIFIED READER. MUTANT HTT STANDARD WORKING SOLUTIONSLysis buffer 4X for two-plate assay protocol on suspension cells Determine the amount of lysis buffer needed for the experiment. Lysates are then transferred to the assay plate for the detection of phospho- CHK2 (Thr 68) by HTRF. Partnerships for delivering turn-key solutions. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. culture plate. • Low volume (20µl) • Compatible with all HTRF readers • For cell-based or biochemical assays Trying is believing. 1) . For a list of HTRF-compatible readers and set-up recommendations, please visit . Manufacturer. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. The Spark multimode reader from Tecan performs accurate and sensitive measurements of fluorescent cell-based assays. For more information about HTRF. Handle complex biological matrices easily. Measure elevated and normal expression samples without multiple dilutions. Using a black. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance for Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. When MikroWin opens, if you already have a pre-existing template for. Configure your microplate reader. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. (donor) of compounds are measured on an HTRF-compatible reader. + 4 µL HTRF pre-mixed antibodies. NC1286225. CLARIOstar is equipped with a specific optical device which enables the measurement of both 620 nm cryptate and 665 nm acceptor emissions. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies Read & analyse Incubate overnight at RT Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. • Clone your plate data for multiple data reduction optionsExpanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™- certified. Read & analyse. Reagents were dispensed following Cisbio’s ligand binding protocol (Figure 3). Envision Nexus. SpectraMax i3/i3x and Paradigm readers can be equipped with. The DLR assay is a two step reaction that uses two luciferase enzymes, Firefly and Renilla (Figure 1). Alpha Technology: The VICTOR Nivo system. The CLARIOstar® Plus is our most flexible multi-mode plate reader. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option. For more information about HTRF. Seal the plate and incubate 1 hour at RT : Remove the plate sealer and read on an HTRF® compatible reader . best fluorescence polarization plate reader on the market. These plates can be read on all HTRF certified readers, thus making their implementation in. • HTRF®-Certified Reader **. For more. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. If not, our scientific team will help you choose the instrument that best suits your needs and budget. HTRF is a proprietary time-resolved fluorescence technology that overcomes Results summary for HTRF IP1 standard curve. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERHTRF Ratio Anti-Tag Antibody Cryptate Inhibitor-Red Inhibitor Purified Tagged Kinase Figure 2: Principle of HTRF kinase competition binding assay (IC 50 - KI determination) HTRF Dasatinib-Red Part # 62KB02REDC & 62KB02REDE Amount: 1 nmol (62KB02REDC) & 20 nmol (62KB02REDE) Concentration: 25 µM in DMSO Form:. The plate map included a low and a high level calibrator, conjugate blanks, and a specific control to assess the counting rate at 620 nm (i. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance for• ®HTRF -Certified Reader(5) (1) IBMX is the most commonly used phosphodiesterase Pan-inhibitor. On the SpectraMax i3x reader, install the HTRF cartridge, and wavelengths will be selected automatically when HTRF is selected as the Optical Configuration. The SpectraMax M5e Reader offers the additional benefit of being certified for Cisbio Bioassays’ HTRF® technology. Even if not listed, some. NON-SPECIFIC BINDING TOTAL BINDING+ 4 µL HTRF pre-mixed antibodies. This protocol gives the cells viability and confluence to be monitored. 96-well culture plateThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. For more information about HTRF. PerkinElmer’s 96w low volume, 96w and 384w microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. Customer Transformation Hub. The donor and acceptor can be used to label a variety of biomolecules, for applications. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. The signals are produced by, converted by, or transmitted through the. The kit includes everything you need: reagents, buffers and a microplate. Even if not listed, some readers may be compatible with HTRF technology. HTRF KinEASE-TK kit and HTRF 96-well low volume plate were obtained from Cisbio Bioassays. Interaction with the industry and research communities is essential for innovating! For more than 30 years, Cisbio has been serving the needs of the clinical and drug discovery communities, delivering a world-class portfolio of assays designed to help find answers faster. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. 5 μL of Binding buffer 2X. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: Small volume detection plate 96-half well plate 25 µL cells + 5 µL compounds + 10 µL supplemented lysis buffer (4X)* + 4 µL HTRF® pre-mixed antibodies Read & analyse Incubate 4h at RT Transfer 16 µL supernatant LysisAdd ONE-PLATE ASSAY PROTOCOL: Small volume detection plate 8 µL cells CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified . For more information about HTRF. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. FOR HTRF CERTIFIED READERThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readers HTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. n HTRF plate validated (QC with reference HTRF assays) n Low to high volumes (10 µL to 350 µL) n Can be used with all HTRF compatible readers n For cell-based or biochemical assays Trying is Believing! Plate Characteristics HTRF® 96 - well low volume plate CulturPlateTM 96 - well Microplate CulturPlate TM 384 - well Microplate ProxiPlateTM 384 - Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Tecan Spark multimode microplate reader, with fluorescence Fusion Optics™ meets these challenges and offers everything you need to develop and run advanced biochemical and cell-based fluorescence assays. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™- certified. (2) Forskolin activates the adenylyl cyclase enzyme and increases the intracellular level of cAMP. The Infinite 200 PRO is an easy-to-use multimode plate reader family that offers affordable, high performance detection solutions, referenced in more than 1800 peer reviewed. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. For a list of HTRF-compatible readers and set-up recommendations, please visit . FOR HTRF CERTIFIED READER For. Learn about the principles, applications, and benefits of HTRF in this Agilent application note. 3 and higher include optimized reader settings, as well as data analysis, for faster results. Plate 25 μL of cells in. Make sure the setup for Tb Cryptate is used. VICTOR® Nivo™ multimode plate reader 2 | For research use only. FOR HTRF CERTIFIED. If not, our scientific team will help you choose the instrument that best suits your needs and budget. For more information about HTRF. Make sure the setup for Tb Cryptate is used. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. Read & analyse. FOR HTRF CERTIFIED READER. use of a wide variety of plates or sample matrix formats. 47 seconds for a 1536-well plate. 96-well culture plate• HTRF®-Certified Reader. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. For a list of HTRF-compatible readers and set-up recommendations, please visit . For more information about HTRF ®The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. 96-well culture plateFOR HTRF CERTIFIED READER. Make sure the setup for Tb Cryptate is used. Read other about this measurement approach here. These plates can be read on all HTRF certified readers, thus making their implementation in. Download Setup. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. 2400 for 665 and 620 with white plate HTRF® Europium cryptate donor / Red acceptor readout. Specialized filters allow for IR fluorescence. The reader only allows high performance for the used HTRF measurement when the HTRF is run with white plates. 96-well or 384-well small volume (SV) detection. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. 5 hours before a further 3 washing steps. FOR HTRF CERTIFIED READER culture plate to a 384-well small volume white plate. It also integrates with the. It measures at any wavelength, with top and bottom reading for most standard technologies. Preconfigured HTRF protocols in SoftMax ® Pro Software 7. * For HTRF microplate recommendations, please visit binding of the fluorescent ligand to the receptor, TR-FRET occurs and is measured with an HTRF-certified microplate reader. PerkinElmer offers two microplate readers certified for use with HTRF: Envision. ratio, high sensitivity, wide dynamic range, superior stability, and excellent flexibility. We would like to show you a description here but the site won’t allow us. HTRF Signal + 5 hours @ RT or . Application Area: absorbance, fluorescence, luminescence, and homogeneous time resolved fluorescence assay development. 3nm, important when reading at 280 nm for DNA analysis, for more exact results. This DLReady-certified microplate reader can also come equipped with Simultaneous Dual Emission for BRET applications and can be further upgraded to a multi-mode reader. Lysates are then transferred to the assay plate for the detection of total-Tau by HTRF. </p> <p>And, the Enhanced Security software option includes tools to facilitate 21 CFR Part 11 compliance for integration into regulated environments (GxP). It accepts all types of microtitration plate. For more information. The multidetection microplate readers CLARIOstar, PHERAstar FS, FLUOstar Omega, and POLARstar Omega are all HTRF-certified by Cisbio, meaning each of the. The LVis Plate is transparent in the UV-range of light and is compatible with all BMG LABTECH microplate readers. Plate Type and Read Area should be selected based on the microplate used and wells to be read. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Eu cryptate emission level).